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We have developed and validated a new two-pass method for fluorescence polarization sequencing for routine clinical analysis. Genomic DNA is first converted to individual complementary DNAs using a thermal program, thereby generating labeled- and unlabeled templates. Each complementary DNA is then converted to a double-stranded DNA probe, which is hybridized to a biotinylated universal primers and extended with Klenow fragment. This is followed by hybridization of the resulting single-stranded DNA to a siliconized streptavidin-coated microparticle. The biotinylated nucleotides are subsequently labeled with a second fluorochrome and the fluorescence polarization measured. DNA sequencing is achieved using conventional capillary electrophoresis with automated fluorescent detection. Several experiments designed to examine the influence of nucleotide base chemistry and the number of misincorporation events were performed. We show that in vitro, the method was robust and that the assay gave excellent, error-free sequence data with a mean read length of 137 +/- 11 bases from a clinical sample.Garden Update - May 13th Hello all! Here is my annual update about what I am doing in the garden this week! As a leader of the garden club and public garden, I am looking at a lot of things with my eyes and sometimes a bit of negative perspective. The current season is the worst one on record. The garden was far below average this year, and there are far more things that I am disappointed about than I am glad about. The first being that we have a lot of volunteer plants in the garden. The zucchini and yellow squash are actually out of this world, from a garden stand point. They are nuts. All the other ones are like 99 cent bugs, and we still had 10x more than last year. It’s a lot! It is a little disappointing because I put a lot of time and energy into figuring out an efficient way to grow my garden. 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